9OWZ

Structure of A. thaliana TBP bound to AG-P nicked DNA site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-08-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	I 1 2 1
Unit cell lengths	122.730, 106.210, 161.922
Unit cell angles	90.00, 101.10, 90.00

Refinement procedure

Resolution	79.660 - 2.910
R-factor	0.2351
Rwork	0.234
R-free	0.25330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.284
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.300	3.100
High resolution limit [Å]	2.910	2.910
Rmerge	0.076	0.595
Rpim	0.049	0.384
Number of reflections	43821	1466
<I/σ(I)>	8.3	1.9
Completeness [%]	97.5
Redundancy	3.3
CC(1/2)	0.994	0.679

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% (w/v) PEG 8000, 0.1 CHES pH 9.5