9OWG
Crystal Structure of the Immunity Protein (52 domain-containing protein) from Pseudomonas aeruginosa PAO1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.27815 |
| Spacegroup name | H 3 |
| Unit cell lengths | 86.425, 86.425, 81.853 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.620 - 1.650 |
| R-factor | 0.17294 |
| Rwork | 0.172 |
| R-free | 0.20051 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.430 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.052 | |
| Rmeas | 0.054 | |
| Rpim | 0.017 | 0.390 |
| Number of reflections | 26908 | 1251 |
| <I/σ(I)> | 42.6 | 1.9 |
| Completeness [%] | 98.0 | 89.6 |
| Redundancy | 10.7 | 9.3 |
| CC(1/2) | 0.997 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | Protein: 8.5 mg/ml, 0.3M Sodium chloride, 0.01M HEPES (pH 7.5); Screen: 0.15M Sodium dihydrogen phosphate, 20% (w/v) PEG 3350; Cryo: glycerol, paratone |
