9OW8

Structure of A. thaliana TBP bound to TBP-TG_P nicked DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	69.117, 117.524, 102.066
Unit cell angles	90.00, 106.94, 90.00

Refinement procedure

Resolution	48.570 - 2.600
R-factor	0.2049
Rwork	0.202
R-free	0.25330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.057
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.600	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.083	0.508
Rpim	0.055	0.321
Number of reflections	46965	4418
<I/σ(I)>	9.6	2.4
Completeness [%]	97.7
Redundancy	3.3
CC(1/2)	0.994	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20% (w/v) PEG 3350, 0.1 M Citric acid pH 5.0 and 0.2 M sodium citrate