9OW7

Structure of A. thaliana TBP bound to TBP-TG nicked DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00004
Spacegroup name	C 2 2 21
Unit cell lengths	77.649, 123.416, 66.381
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.720 - 2.300
R-factor	0.1911
Rwork	0.189
R-free	0.22960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.006
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.720	2.450
High resolution limit [Å]	2.300	2.300
Rmerge	0.053	0.644
Rpim	0.027	0.350
Number of reflections	17896	328
<I/σ(I)>	13.6	1.4
Completeness [%]	98.6
Redundancy	5.8
CC(1/2)	0.998	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	4 M sodium Formate, 0.1 M sodium acetate pH 3.8.