9OVZ

Gallid alphaherpesvirus-2 large tegument protein NLS in complex with Importin alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-08-22
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.95373
Spacegroup name	P 21 21 21
Unit cell lengths	77.881, 91.064, 99.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.100 - 2.400
R-factor	0.211
Rwork	0.210
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.488
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.066	0.795
Rmeas		0.228
Rpim	0.019
Number of reflections	28404	2777
<I/σ(I)>	25.74	1.85
Completeness [%]	99.9	99.82
Redundancy	13.5
CC(1/2)	0.997	0.918

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	296	0.7 M sodium citrate, 0.1 M HEPES, 0.01 M DTT, pH 7.0