Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 201.936, 51.254, 76.314 |
| Unit cell angles | 90.00, 106.47, 90.00 |
Refinement procedure
| Resolution | 30.510 - 2.175 |
| R-factor | 0.2207 |
| Rwork | 0.218 |
| R-free | 0.26940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 96.827 |
| High resolution limit [Å] | 2.175 |
| Rmerge | 0.059 |
| Rmeas | 0.071 |
| Rpim | 0.039 |
| Number of reflections | 19849 |
| <I/σ(I)> | 7.6 |
| Completeness [%] | 99.4 |
| Redundancy | 3.3 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 293 | 0.1 M magnesium acetate, 50 mM MES pH 5.5, and 9-16% 2-methyl 2,4-pentane diol |
