9OV2

Crystal structure of human IGA2M1 FC fragment-FC-alpha receptor (CD89) complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-05-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 21
Unit cell lengths	46.683, 129.553, 183.343
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.910 - 2.300
R-factor	0.215
Rwork	0.213
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.815
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.127
Rpim	0.056	0.578
Number of reflections	24454	1260
<I/σ(I)>	29.9	2.8
Completeness [%]	96.2	99.9
Redundancy	6.2	6.3
CC(1/2)	0.990	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	0.15 M sodium chloride 0.1 M Tris-HCl pH 8.0 15% PEG 1000