9OUH
K-Ras G12D Cysteine Light at 293 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE 7B2 |
| Synchrotron site | CHESS |
| Beamline | 7B2 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2023-10-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.734, 51.871, 68.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.330 - 1.400 |
| R-factor | 0.2008 |
| Rwork | 0.199 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.190 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.330 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmeas | 0.078 | 0.306 |
| Rpim | 0.024 | 0.110 |
| Number of reflections | 36777 | 1795 |
| <I/σ(I)> | 288.8 | 20.4 |
| Completeness [%] | 99.3 | |
| Redundancy | 10.5 | |
| CC(1/2) | 0.999 | 0.957 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG8000, 200 mM sodium acetate, 100 mM ammonium acetate |
