9OTR
Crystal Structure of Salmonella FraB Deglycase, Crystal Form 4 with deletion of C-terminal residues 313-325.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979330 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.482, 108.363, 119.151 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.434 - 1.839 |
| Rwork | 0.172 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.684 |
| Data reduction software | XDS (Feb 5, 2021 (BUILT 20210205)) |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.434 | 1.987 |
| High resolution limit [Å] | 1.839 | 1.839 |
| Rmerge | 0.065 | 0.919 |
| Rpim | 0.041 | |
| Number of reflections | 45084 | 2254 |
| <I/σ(I)> | 16.7 | 1.8 |
| Completeness [%] | 71.2 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% w/v PEG3350, 0.2 M sodium sulfate, 0.1 M Bis-Tris propane, pH 8.0-8.5 |
