9OT9
Crystal structure of the Galectin-3 complex with FN3con-7, an mRNA display-derived binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.703, 51.237, 107.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.220 - 1.923 |
| R-factor | 0.267 |
| Rwork | 0.265 |
| R-free | 0.31350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.220 | 2.070 |
| High resolution limit [Å] | 1.923 | 1.923 |
| Rpim | 0.034 | 0.571 |
| Number of reflections | 17748 | 887 |
| <I/σ(I)> | 12.9 | 1.3 |
| Completeness [%] | 93.4 | 43.2 |
| Redundancy | 13 | 8.1 |
| CC(1/2) | 0.999 | 0.484 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25 % (w/v) PEG 3350, 0.1 M HEPES pH 7.5 |
