9OT0
Antarctic Rhodopsin crystallized in LCP at pH 4.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 107.339, 107.339, 50.644 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.870 - 2.070 |
| R-factor | 0.2106 |
| Rwork | 0.207 |
| R-free | 0.23880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.954 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.870 | 2.130 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Number of reflections | 20493 | 1586 |
| <I/σ(I)> | 8.3 | 1 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 4.9 | 5 |
| CC(1/2) | 0.992 | 0.418 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 4.6 | 293.15 | 0.1 M sodium acetate at pH 4.6, 0.02 M calcium chloride, 15% w/v 2-methyl-2,4-pentanediol |
