9OSX

PfCyRPA-EM epitope mimic bound to monoclonal antibody Cy.004

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-03
Detector	DECTRIS EIGER2 XE 9M
Wavelength(s)	0.92124
Spacegroup name	C 1 2 1
Unit cell lengths	87.615, 37.976, 173.374
Unit cell angles	90.00, 92.47, 90.00

Refinement procedure

Resolution	173.210 - 2.670
R-factor	0.2556
Rwork	0.254
R-free	0.29210
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	173.210	2.700
High resolution limit [Å]	2.650	2.650
Number of reflections	16178	538
<I/σ(I)>	4.1
Completeness [%]	95.0
Redundancy	3.4
CC(1/2)	0.970	0.335

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M sodium acetate, pH 4.0, 0.2 M ammonium acetate, 15% w/v PEG4000