9OSA
Crystal Strucutre of SwnH1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-04 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 265.541, 151.284, 187.633 |
| Unit cell angles | 90.00, 134.17, 90.00 |
Refinement procedure
| Resolution | 32.730 - 2.810 |
| R-factor | 0.2238 |
| Rwork | 0.221 |
| R-free | 0.26650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.074 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 125833 | 125832 |
| <I/σ(I)> | 0.4 | |
| Completeness [%] | 96.9 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.964 | 0.964 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Magnesium acetate tetrahydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 20%w/v Polyethylene glycol 8000 |
