9ORW
X-ray diffraction structure of apo-form lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2025-05-19 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.900, 76.900, 37.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.380 - 1.500 |
| R-factor | 0.1836 |
| Rwork | 0.182 |
| R-free | 0.21840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.380 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.049 | 0.234 |
| Number of reflections | 34094 | 6013 |
| <I/σ(I)> | 38.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.6 | 13.3 |
| CC(1/2) | 1.000 | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 800 mM NaCl, 80 mM sodium acetate pH 4.8 |
