9ORK

Antarctic Rhodopsin crystallized in LCP at pH 8.5

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920
Spacegroup name	P 3 2 1
Unit cell lengths	107.613, 107.613, 50.617
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.910 - 2.590
R-factor	0.1938
Rwork	0.191
R-free	0.21830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.002
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.910	2.660
High resolution limit [Å]	2.590	2.590
Number of reflections	10412	738
<I/σ(I)>	13.2	1.8
Completeness [%]	97.9	93.8
Redundancy	5.2	4.9
CC(1/2)	0.999	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	8.5	279.15	0.2 M NaCl, 20% w/v 2-methyl-2,4-pentanediol, 4% w/v glycerol