9OR2

Antarctic Rhodopsin crystallized in bicelles at pH 4.0

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033
Spacegroup name	P 3 2 1
Unit cell lengths	108.356, 108.356, 54.733
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.760 - 2.070
R-factor	0.2023
Rwork	0.201
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.357
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.760	2.130
High resolution limit [Å]	2.070	2.070
Number of reflections	22686	1796
<I/σ(I)>	9.9	1
Completeness [%]	99.4	99.9
Redundancy	16.5	14.7
CC(1/2)	0.998	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	307.15	3.2 M - 3.6 M sodium phosphate monobasic monohydrate, pH 4.0, 180 mM 1,6-hexanediol, 3.5% triethylene glycol