9OO2

N-terminal Six-His-Tagged FosB from Enterococcus faecium in complex with fosfomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	75.050, 75.050, 116.265
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.070 - 2.200
R-factor	0.1926
Rwork	0.189
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.917
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.070	2.110
High resolution limit [Å]	2.090	2.090
Number of reflections	82848	1671
<I/σ(I)>	3.55
Completeness [%]	100.0
Redundancy	21.7
CC(1/2)	0.995	0.062

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, magnesium chloride