9ONY

N-terminal Six-His-Tagged FosB from Enterococcus faecium in complex with (1-Hydroxypropan-2-yl)phosphonic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-04-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	74.551, 74.551, 107.282
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	61.220 - 2.500
R-factor	0.2452
Rwork	0.242
R-free	0.27280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.522
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.220	2.500
High resolution limit [Å]	2.470	2.470
Number of reflections	76584	94
<I/σ(I)>	-0.18
Completeness [%]	98.5
Redundancy	20.7
CC(1/2)	0.824	0.311

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, magnesium chloride