9ONW

N-terminal Six-His-Tagged FosB from Enterococcus faecium in complex with (1-Hydroxy-2-methylpropyl)phosphonic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	DIFFRACTOMETER
Collection date	2025-04-01
Detector	RIGAKU
Wavelength(s)	1.54
Spacegroup name	P 41 21 2
Unit cell lengths	76.211, 76.211, 119.138
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.740 - 2.800
R-factor	0.2952
Rwork	0.292
R-free	0.32460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.503
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.740	2.730
High resolution limit [Å]	2.700	2.700
Number of reflections	24224	765
<I/σ(I)>	1.46
Completeness [%]	94.3
Redundancy	5.2
CC(1/2)	0.837	0.116

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, magnesium chloride