9ONV
FosB from Enterococcus faecium in complex with phosphonoformate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2025-02-27 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 83.574, 83.574, 104.242 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.430 - 2.200 |
| R-factor | 0.1918 |
| Rwork | 0.188 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.901 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.430 | 2.270 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.123 | 0.752 |
| Rmeas | 0.134 | 0.821 |
| Rpim | 0.053 | 0.321 |
| Number of reflections | 24283 | 8145 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 99.9 | 99.78 |
| Redundancy | 6.3 | 5.3 |
| CC(1/2) | 0.993 | 0.880 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 400, HEPES Buffer, Ammonium Sulfate, Magnesium Chloride |
