9OMN
WrtF fucosyltransferase - GDP-2-deoxy-2-fluoro-beta-L-fucose
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.181 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.530, 124.330, 45.770 |
| Unit cell angles | 90.00, 93.95, 90.00 |
Refinement procedure
| Resolution | 45.660 - 2.300 |
| R-factor | 0.2137 |
| Rwork | 0.210 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.544 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.660 | 2.382 |
| High resolution limit [Å] | 2.070 | 2.300 |
| Number of reflections | 29467 | 2115 |
| <I/σ(I)> | 9.1 | 1.92 |
| Completeness [%] | 99.7 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.998 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 200 mM ammonium sulfate, 100 mM HEPES pH 7.5, 35% poly (acrylic acid sodium salt) 2100 |
