9OMJ
WrtF fucosyltransferase - GDP-beta-L-fucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-04 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.181 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.910, 65.640, 124.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.150 - 1.600 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.434 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.150 | 1.657 |
| High resolution limit [Å] | 1.480 | 1.600 |
| Number of reflections | 87116 | 6960 |
| <I/σ(I)> | 13 | 1.28 |
| Completeness [%] | 97.8 | 99.86 |
| Redundancy | 9.2 | 9.8 |
| CC(1/2) | 0.999 | 0.631 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 200 mM ammonium sulfate, 100 mM HEPES pH 7.5, 35% poly (acrylic acid sodium salt) 2100 |
