9OMI
WrtF fucosyltransferase - GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-04 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.181 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.830, 65.960, 123.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.240 - 1.970 |
| R-factor | 0.1898 |
| Rwork | 0.187 |
| R-free | 0.23420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.768 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.240 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 37512 | 3219 |
| <I/σ(I)> | 14.1 | 1.41 |
| Completeness [%] | 98.3 | |
| Redundancy | 8.9 | 5.6 |
| CC(1/2) | 0.999 | 0.592 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 200 mM ammonium sulfate, 100 mM HEPES pH 7.5, 35% poly (acrylic acid sodium salt) 2100 |
