9OMH
WrtF fucosyltransferase - Apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.180, 124.290, 64.770 |
| Unit cell angles | 90.00, 93.00, 90.00 |
Refinement procedure
| Resolution | 46.770 - 1.450 |
| Rwork | 0.165 |
| R-free | 0.19080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.671 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.770 | 1.502 |
| High resolution limit [Å] | 1.350 | 1.450 |
| Number of reflections | 216717 | 17844 |
| <I/σ(I)> | 8.5 | 0.85 |
| Completeness [%] | 97.9 | 99.88 |
| Redundancy | 6.5 | 6.5 |
| CC(1/2) | 0.998 | 0.435 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 200 mM ammonium sulfate, 100 mM HEPES pH 7.5, 35% poly (acrylic acid sodium salt) 2100 |
