9OLU

GX/NPIH26 bat norovirus protruding domain in complex with L-fucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95366
Spacegroup name	P 32 2 1
Unit cell lengths	67.542, 67.542, 238.385
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.960 - 1.550
R-factor	0.1588
Rwork	0.158
R-free	0.19370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.253
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.680	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.065	1.417
Rmeas	0.068	1.477
Rpim	0.019	0.407
Total number of observations	1225877	54189
Number of reflections	91925	4332
<I/σ(I)>	16.5	1.4
Completeness [%]	99.8
Redundancy	13.3	12.5
CC(1/2)	1.000	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	1.6 M magnesium sulfate heptahydrate, 0.1 M MES (pH 6.5)