9OLT
GII.27: Loreto0959 norovirus protruding domain complexed with A-trisaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.939, 90.695, 66.786 |
| Unit cell angles | 90.00, 106.04, 90.00 |
Refinement procedure
| Resolution | 45.450 - 1.760 |
| R-factor | 0.1503 |
| Rwork | 0.149 |
| R-free | 0.19640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.945 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.450 | 1.800 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.060 | 0.533 |
| Rmeas | 0.064 | 0.568 |
| Rpim | 0.022 | 0.194 |
| Total number of observations | 469281 | 24899 |
| Number of reflections | 57076 | 3058 |
| <I/σ(I)> | 16.8 | 2.8 |
| Completeness [%] | 99.0 | |
| Redundancy | 8.2 | 8.1 |
| CC(1/2) | 1.000 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.17 M Ammonium sulfate, 25.5% w/v PEG 4000 |
