9OKY

16mer self-complementary duplex RNA with dG:s(2)C pair sequence 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	99
Detector technology	PIXEL
Collection date	2024-06-28
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97648
Spacegroup name	H 3 2
Unit cell lengths	43.422, 43.422, 256.437
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.326 - 1.478
Rwork	0.220
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.212
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.510
High resolution limit [Å]	1.478	1.478
Rmerge	0.070	0.506
Rmeas	0.075	0.562
Rpim	0.025	0.237
Number of reflections	15145	756
<I/σ(I)>	20.2	2.7
Completeness [%]	93.2	93.6
Redundancy	8.2	5
CC(1/2)	0.989	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.1 M BIS-TRIS pH 5.5, 2.0 M Ammonium sulfate