9OKX

16mer self-complementary duplex RNA with dG:C pair sequence 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	99
Detector technology	PIXEL
Collection date	2024-06-28
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97648
Spacegroup name	H 3 2
Unit cell lengths	41.511, 41.511, 124.629
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.543 - 1.375
Rwork	0.177
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.859
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.400
High resolution limit [Å]	1.375	1.375
Rmerge	0.067	0.494
Rmeas	0.072	0.548
Rpim	0.027	0.232
Number of reflections	8922	405
<I/σ(I)>	26	2.8
Completeness [%]	99.5	96.2
Redundancy	7.2	5.2
CC(1/2)	0.972	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	293	0.4 M Sodium chloride, 0.12 M Calcium chloride, 20 mM MES pH 5.8, 27% v/v (+/-)-2-Methyl-2,4-pentanediol