9OKU

16mer self-complementary duplex RNA with dA:s(2)U pair sequence 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2024-10-30
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	H 3 2
Unit cell lengths	41.167, 41.167, 124.563
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.521 - 1.479
Rwork	0.170
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.982
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.510
High resolution limit [Å]	1.479	1.479
Rmerge	0.061	0.573
Rmeas	0.065	0.609
Rpim	0.022	0.204
Number of reflections	7122	344
<I/σ(I)>	33	3.5
Completeness [%]	100.0	100
Redundancy	9.4	8.6
CC(1/2)	1.000	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, 30% v/v Polyethylene glycol 400