9OKT

16mer self-complementary duplex RNA with dA:U pair sequence 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2024-12-22
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	H 3 2
Unit cell lengths	41.006, 41.006, 123.438
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.128 - 1.320
Rwork	0.183
R-free	0.20650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	2.297
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.340
High resolution limit [Å]	1.320	1.320
Rmerge	0.060	0.511
Rmeas	0.064	0.543
Rpim	0.021	0.180
Number of reflections	9461	417
<I/σ(I)>	30.3	4
Completeness [%]	96.9	88.7
Redundancy	9.4	8.6
CC(1/2)	0.992	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.08 M Sodium chloride, 0.02 M Magnesium chloride hexahydrate, 0.04 M Sodium cacodylate trihydrate pH 5.5, 35% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.002 M Hexammine cobalt(III) chloride