9OJV

Crystal structure of monoclonal J2 antibody bound to dsRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	65.391, 92.374, 194.663
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.640 - 2.850
R-factor	0.2139
Rwork	0.212
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.443
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.640	2.960
High resolution limit [Å]	2.850	2.850
Rmerge	0.452	2.770
Rpim	0.085	0.540
Number of reflections	28092	3013
<I/σ(I)>	7.3	1
Completeness [%]	99.5
Redundancy	29.2
CC(1/2)	0.992	0.444

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	0.15 M malic acid pH 7, 0.1 M imidazole, 22% v/v PEG MME 550