9OJ4
Mouse LRRC15 extracellular domain in complex with disulfide constrained peptide ML-YSD-07 and Fab-E1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-23 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97737 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 209.140, 66.601, 92.532 |
| Unit cell angles | 90.00, 111.58, 90.00 |
Refinement procedure
| Resolution | 36.910 - 2.570 |
| R-factor | 0.1894 |
| Rwork | 0.187 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.561 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3546) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.910 | 2.661 |
| High resolution limit [Å] | 2.569 | 2.569 |
| Rmerge | 0.124 | 0.814 |
| Rmeas | 0.137 | 0.896 |
| Number of reflections | 35844 | 3477 |
| <I/σ(I)> | 10.41 | 2.24 |
| Completeness [%] | 94.0 | 91.76 |
| Redundancy | 5.6 | 5.6 |
| CC(1/2) | 0.996 | 0.832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1 M Tris pH 8.5, 10% w/v PEG8K |
