9OIB

Mouse LRRC15 extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00004
Spacegroup name	P 21 21 21
Unit cell lengths	45.066, 90.693, 103.713
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.549 - 2.260
R-factor	0.2106
Rwork	0.207
R-free	0.24480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.505
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.16_3546: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.550	2.341
High resolution limit [Å]	2.260	2.260
Rmerge	0.103	0.846
Rmeas	0.112
Number of reflections	20546	1978
<I/σ(I)>	13.66	2.23
Completeness [%]	99.8	99.85
Redundancy	6.5
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	292	0.1 M Sodium malonate pH 6.0, 12% w/v PEG3350