Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.91683 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.948, 68.791, 107.456 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.940 - 1.660 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.23860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.184 |
| Data reduction software | autoPROC (1.1.7) |
| Data scaling software | Aimless (0.7.15) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.940 | 1.750 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.125 | 1.869 |
| Rmeas | 0.131 | 2.020 |
| Rpim | 0.037 | 0.748 |
| Total number of observations | 644704 | 37611 |
| Number of reflections | 54814 | 6141 |
| <I/σ(I)> | 12.3 | 1 |
| Completeness [%] | 94.7 | |
| Redundancy | 11.8 | 6.1 |
| CC(1/2) | 0.999 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290.15 | 18.0% w/v PEG 3350, 0.18 M Tri-ammonium citrate |
