9OFR

CRYSTAL STRUCTURE OF THE HUMAN IGA1 FC FRAGMENT-FC-ALPHA RECEPTOR (CD89) COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-08-02
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	63.301, 176.778, 100.019
Unit cell angles	90.00, 96.10, 90.00

Refinement procedure

Resolution	40.390 - 2.650
R-factor	0.2159
Rwork	0.213
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.787
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.160
Rpim	0.096	0.505
Number of reflections	60192	2536
<I/σ(I)>	16.5	1.5
Completeness [%]	94.7	80.8
Redundancy	3.6	2.7
CC(1/2)	0.948	0.517

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	0.15M sodium chloride 0.1M Tris-HCl pH 8.0 8% PEG 6000