9OEX
K-Ras G12V at 293 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE 7B2 |
| Synchrotron site | CHESS |
| Beamline | 7B2 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2022-03-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.757, 42.530, 116.353 |
| Unit cell angles | 90.00, 105.20, 90.00 |
Refinement procedure
| Resolution | 37.430 - 1.500 |
| R-factor | 0.203 |
| Rwork | 0.202 |
| R-free | 0.22080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.270 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.430 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.062 | 0.927 |
| Rpim | 0.036 | 0.557 |
| Number of reflections | 48752 | 3872 |
| <I/σ(I)> | 107 | 2.8 |
| Completeness [%] | 96.2 | |
| Redundancy | 1.5 | |
| CC(1/2) | 1.000 | 0.709 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM sodium acetate, pH 4.5-4.7, 100 mM Tris, pH 8.5, 20% PEG3350, 1 mM DTT |
