9OEL
KRAS Wild Type 1-169 at 293 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE 7B2 |
| Synchrotron site | CHESS |
| Beamline | 7B2 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2022-03-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 66.567, 42.773, 116.116 |
| Unit cell angles | 90.00, 105.16, 90.00 |
Refinement procedure
| Resolution | 37.360 - 1.500 |
| R-factor | 0.1912 |
| Rwork | 0.190 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.088 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.430 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmeas | 0.650 | |
| Rpim | 0.036 | 0.210 |
| Number of reflections | 50706 | 4971 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 96.2 | |
| Redundancy | 9.2 | |
| CC(1/2) | 0.992 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM sodium acetate, pH 3.6-5.6, 100 mM Tris, pH 8.5, 22-28% PEG3350 |
