9OD7

Structure of disulfide-stabilized IL-18 variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-26
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97946
Spacegroup name	P 61
Unit cell lengths	70.916, 70.916, 89.904
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.270 - 1.900
R-factor	0.1593
Rwork	0.158
R-free	0.19540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.965
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1-4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.712	1.928
High resolution limit [Å]	1.895	1.895
Number of reflections	18169	1017
<I/σ(I)>	24.3
Completeness [%]	89.1
Redundancy	9.7
CC(1/2)	1.000	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		273.15	0.1M MIB buffer (mixing sodium malonate, imidazole, and boric acid in the molar ratios 2:3:3), pH 4.0, 25% PEG 1500