9OBR
Dye-decolorizing peroxidase from Acinetobacter baumannii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2025-03-05 |
| Detector | BRUKER PHOTON 100 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.263, 141.251, 86.993 |
| Unit cell angles | 90.00, 102.88, 90.00 |
Refinement procedure
| Resolution | 23.150 - 2.600 |
| R-factor | 0.1961 |
| Rwork | 0.195 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.163 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5134) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.150 | 2.630 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.130 | 0.430 |
| Number of reflections | 99266 | 6641 |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 98.0 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100 mM CAPS, pH 10.5, 20% PEG8000, 200 mM sodium chloride |
