9OBE
Crystal structure of human Argonaute2 in complex with a fully modified siRNA with a 5'-phenylpropyl phosphate
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-09-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.116, 108.236, 68.529 |
| Unit cell angles | 90.00, 107.59, 90.00 |
Refinement procedure
| Resolution | 60.170 - 2.300 |
| R-factor | 0.2087 |
| Rwork | 0.206 |
| R-free | 0.25240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.736 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.170 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 75662 | 3845 |
| <I/σ(I)> | 19.01 | 4.09 |
| Completeness [%] | 98.7 | 99.61 |
| Redundancy | 2 | |
| CC(1/2) | 0.999 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG3350, Tris-HCl pH8, 2-propanol, phenol |
