9OB7
Crystal structure of delipidated human fatty acid binding protein 4 (FABP4) in the apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-01 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.319, 53.617, 74.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.230 - 1.300 |
| R-factor | 0.1811 |
| Rwork | 0.180 |
| R-free | 0.19290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.839 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.950 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.053 | 0.586 |
| Rmeas | 0.056 | 0.686 |
| Rpim | 0.016 | 0.346 |
| Total number of observations | 337962 | 4177 |
| Number of reflections | 32446 | 1367 |
| <I/σ(I)> | 24.8 | 2 |
| Completeness [%] | 99.3 | |
| Redundancy | 10.4 | 3.1 |
| CC(1/2) | 1.000 | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.55-1.6 M sodium citrate |
