9OAX

TNA polymerase, 5-270, ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2023-06-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	77.441, 101.713, 110.847
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.670 - 3.030
R-factor	0.2294
Rwork	0.224
R-free	0.27770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.381
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.670	3.140
High resolution limit [Å]	3.030	3.030
Rmerge	0.163	1.017
Number of reflections	17539	1729
<I/σ(I)>	12.57
Completeness [%]	99.6
Redundancy	6.6
CC(1/2)	0.995	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2M sodium iodide, 20% PEG 3350, 0.1 M calcium chloride dihydrate