9OA7

GNAT family acetyltransferase EryM SeMet

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 3
Unit cell lengths	152.940, 152.940, 152.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.360 - 2.600
R-factor	0.1909
Rwork	0.189
R-free	0.23410
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.048
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.360	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.118	1.473
Number of reflections	1328601	7096
<I/σ(I)>	19.5
Completeness [%]	100.0
Redundancy	21
CC(1/2)	0.999	0.754

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277.15	1.8 M (NH4)2SO4, 3% (w/v) 2-methyl-2,4-pentanediol, 0.2 M NaCl, 0.1 M sodium cacodylate, pH 6