9OA4
Human PU.1 ETS-Domain (165-270) Bound to d(5'-AATAAGCGGAAGTGGG-3') d(5'-TCCCACTTTCGCTTAT-3') with a GT mismatch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920119 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.134, 60.695, 44.802 |
| Unit cell angles | 90.00, 116.59, 90.00 |
Refinement procedure
| Resolution | 24.190 - 1.420 |
| R-factor | 0.161 |
| Rwork | 0.159 |
| R-free | 0.19460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.037 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.190 | 1.471 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.043 | 0.428 |
| Rmeas | 0.049 | 0.487 |
| Rpim | 0.024 | 0.228 |
| Number of reflections | 38841 | 3882 |
| <I/σ(I)> | 16.66 | 2.89 |
| Completeness [%] | 99.3 | 99.67 |
| Redundancy | 4.3 | 4.3 |
| CC(1/2) | 0.997 | 0.926 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 2% PEG 3350, 100 mM Sodium Acetate, pH 4.6 |
