Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 42.899, 42.899, 172.743 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.320 - 2.350 |
| R-factor | 0.1951 |
| Rwork | 0.189 |
| R-free | 0.25280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.056 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.150 | 2.180 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.090 | 0.976 |
| Rmeas | 0.101 | 1.102 |
| Rpim | 0.045 | 0.503 |
| Total number of observations | 54772 | 3758 |
| Number of reflections | 11234 | 829 |
| <I/σ(I)> | 7.1 | 0.8 |
| Completeness [%] | 99.3 | |
| Redundancy | 4.9 | 4.5 |
| CC(1/2) | 0.997 | 0.460 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 0.1 M Tris-HCl pH 8.5, 0.2 M MgCl2, 30% PEG 4000 |
