9O9B

Structure of the IL-18 Surrogate Cytokine Agonist DR3097

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 3
Unit cell lengths	120.100, 120.100, 29.268
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.310 - 2.800
R-factor	0.2461
Rwork	0.233
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.331
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.310	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.112	0.672
Rmeas	0.140	0.838
Rpim	0.082	0.489
Total number of observations	31453	4688
Number of reflections	11208	1667
<I/σ(I)>	6.7	1.6
Completeness [%]	96.3
Redundancy	2.8	2.8
CC(1/2)	0.984	0.609

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	3.6 M sodium formate 10% (v/v) glycerol