9O99
Structure of the IL-2 Surrogate Cytokine Agonist DR638
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-12-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.205, 63.135, 87.451 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.800 - 1.800 |
| R-factor | 0.18164 |
| Rwork | 0.180 |
| R-free | 0.21114 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.767 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.810 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.067 | 0.774 |
| Rmeas | 0.077 | 0.889 |
| Rpim | 0.036 | 0.430 |
| Total number of observations | 115493 | 6709 |
| Number of reflections | 25844 | 1491 |
| <I/σ(I)> | 12.8 | 3.1 |
| Completeness [%] | 99.6 | |
| Redundancy | 4.5 | 4.5 |
| CC(1/2) | 0.998 | 0.749 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10% (v/v) glycerol, 0.1 M HEPES pH 7.5, 30% (v/v) PEG 400 and 5% (w/v) PEG 3000 |
