9O8V

Apo Form of Photoactive Yellow Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-03-06
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	104.631, 50.723, 71.252
Unit cell angles	90.00, 132.93, 90.00

Refinement procedure

Resolution	38.300 - 1.290
R-factor	0.1594
Rwork	0.158
R-free	0.18530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.352
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.320
High resolution limit [Å]	1.290	3.530	1.300
Rmerge	0.065	0.048	0.708
Rmeas	0.073	0.054	0.811
Rpim	0.033	0.024	0.389
Number of reflections	66798	3451	3224
<I/σ(I)>	13
Completeness [%]	98.5	98.5	95.2
Redundancy	4.7	4.8	4
CC(1/2)	1.000	0.996	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	Ammonium Sulfate, Sodium chloride, Potassium phosphate