9O8K

[6,8,5] Shifted tensegrity triangle with an (arm, center, arm) distribution of (6, 8, 5) base pairs, 2 nt sticky ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-24
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.007430
Spacegroup name	P 63
Unit cell lengths	130.562, 130.562, 54.231
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.640 - 6.050
R-factor	0.1567
Rwork	0.156
R-free	0.17550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.870
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.281	65.281	6.756
High resolution limit [Å]	6.050	12.016	6.052
Number of reflections	1131	187	188
<I/σ(I)>	17
Completeness [%]	90.0
Redundancy	19.4
CC(1/2)	1.000	1.000	0.373

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate