9O8I

[3,8,8,P-2] Shifted tensegrity triangle with an (arm, center, arm) distribution of (3, 8, 8) base pairs, 2 nt sticky ends, and 5' phosphates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.991870
Spacegroup name	H 3
Unit cell lengths	110.293, 110.293, 67.153
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.840 - 3.170
R-factor	0.2234
Rwork	0.221
R-free	0.25730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.713
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.147	55.146	3.485
High resolution limit [Å]	3.166	9.320	3.166
Number of reflections	3450	203	204
<I/σ(I)>	6.9
Completeness [%]	80.9
Redundancy	9
CC(1/2)	0.998	0.998	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate